RAYNE, Fabienne; WITTKOP, Linda; BADER, Clement; KASSAB, Somar; TUMIOTTO, Camille; BERCIAUD, Sylvie; WODRICH, Harald; LAFON, Marie-Edith

doi:10.1016/j.jviromet.2017.09.009

Microbiologie Fondamentale et Pathogénicité [MFP]

WITTKOP, Linda
Bordeaux population health [BPH]

BADER, Clement

Idioma

Article de revue

Este ítem está publicado en

Journal of Virological Methods. 2017-11-01, vol. 249, p. 156-160

Resumen en inglés

Adenoviruses are characterized by a large variability, reflected by their classification in species A to G. Certain species, eg A and C, could be associated with increased clinical severity, both in immunocompetent and immunocompromised hosts suggesting that in some instances species identification provides clinically relevant information. Here we designed a novel "pVI rapid typing method" to obtain quick, simple and cost effective species assignment for Adenoviruses, thanks to combined fusion temperature (Tm) and amplicon size analysis. Rapid typing results were compared to Sanger sequencing in the hexon gene for 140 Adenovirus-positive clinical samples included in the Typadeno study. Species A and C could be identified with a 100% positive predictive value, thus confirming the value of this simple typing method.< Leer menos

Palabras clave en inglés

Adenovirus Infections

Human

Adenoviruses

Human

DNA Primers

Genotyping Techniques

Humans

Immunocompetence

Immunocompromised Host

Polymerase Chain Reaction

Predictive Value of Tests

Sensitivity and Specificity

Sequence Analysis

DNA

Transition Temperature

URI

https://oskar-bordeaux.fr/handle/20.500.12278/184739

DOI

http://dx.doi.org/10.1016/j.jviromet.2017.09.009

Centros de investigación

MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234

Ver/Abrir

Metadatos

Compartir este ítem

Licencia de uso del documento

Rapid Adenovirus typing method for species identification.

Idioma

Este ítem está publicado en

Resumen en inglés

Palabras clave en inglés

URI

DOI

Centros de investigación