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Rapid Adenovirus typing method for species identification.
Langue
EN
Article de revue
Ce document a été publié dans
Journal of Virological Methods. 2017-11-01, vol. 249, p. 156-160
Résumé en anglais
Adenoviruses are characterized by a large variability, reflected by their classification in species A to G. Certain species, eg A and C, could be associated with increased clinical severity, both in immunocompetent and ...Lire la suite >
Adenoviruses are characterized by a large variability, reflected by their classification in species A to G. Certain species, eg A and C, could be associated with increased clinical severity, both in immunocompetent and immunocompromised hosts suggesting that in some instances species identification provides clinically relevant information. Here we designed a novel "pVI rapid typing method" to obtain quick, simple and cost effective species assignment for Adenoviruses, thanks to combined fusion temperature (Tm) and amplicon size analysis. Rapid typing results were compared to Sanger sequencing in the hexon gene for 140 Adenovirus-positive clinical samples included in the Typadeno study. Species A and C could be identified with a 100% positive predictive value, thus confirming the value of this simple typing method.< Réduire
Mots clés en anglais
Adenovirus Infections
Human
Adenoviruses
Human
DNA Primers
Genotyping Techniques
Humans
Immunocompetence
Immunocompromised Host
Polymerase Chain Reaction
Predictive Value of Tests
Sensitivity and Specificity
Sequence Analysis
DNA
Transition Temperature
Unités de recherche