BROSTER REIX, Christine; FLORIMOND, Célia; CAYREL, Anne; MAILHÉ, Amélie; AGNERO-RIGOT, Corentin; LANDREIN, Nicolas; DACHEUX, Denis; HAVLICEK, Katharina; BONHIVERS, Melanie; MORRISWOOD, Brooke; ROBINSON, Derrick

doi:10.3390/microorganisms9112334

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BROSTER REIX, Christine
Microbiologie Fondamentale et Pathogénicité [MFP]

FLORIMOND, Célia
Microbiologie Fondamentale et Pathogénicité [MFP]

CAYREL, Anne
Microbiologie Fondamentale et Pathogénicité [MFP]

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Article de revue

Ce document a été publié dans

Microorganisms. 2021-11-11, vol. 9, n° 11, p. 2334

Résumé en anglais

Background: In most trypanosomes, endo and exocytosis only occur at a unique organelle called the flagellar pocket (FP) and the flagellum exits the cell via the FP. Investigations of essential cytoskeleton-associated structures located at this site have revealed a number of essential proteins. The protein TbBILBO1 is located at the neck of the FP in a structure called the flagellar pocket collar (FPC) and is essential for biogenesis of the FPC and parasite survival. TbMORN1 is a protein that is present on a closely linked structure called the hook complex (HC) and is located anterior to and overlapping the collar. TbMORN1 is essential in the bloodstream form of T. brucei. We now describe the location and function of BHALIN, an essential, new FPC-HC protein. Methodology/Principal Findings: Here, we show that a newly characterised protein, BHALIN (BILBO1 Hook Associated LINker protein), is localised to both the FPC and HC and has a TbBILBO1 binding domain, which was confirmed in vitro. Knockdown of BHALIN by RNAi in the bloodstream form parasites led to cell death, indicating an essential role in cell viability. Conclusions/Significance: Our results demonstrate the essential role of a newly characterised hook complex protein, BHALIN, that influences flagellar pocket organisation and function in bloodstream form T. brucei parasites.< Réduire

Mots clés en anglais

cytoskeleton

endocytosis

flagellar pocket

flagellar pocket collar

hook complex

protozoan

trypanosoma

URI

https://oskar-bordeaux.fr/handle/20.500.12278/182218

DOI

http://dx.doi.org/10.3390/microorganisms9112334

Project ANR

Pourquoi et comment les trypanosomes construisent un Collier de la Poche Flagellaire - ANR-20-CE91-0003
Alliance française contre les maladies parasitaires

Unités de recherche

MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234