Bhalin, an essential cytoskeleton-associated protein of $Trypanosoma\ brucei$ linking TbBILBO1 of the flagellar pocket collar with the hook complex
| dc.rights.license | open | en_US |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | BROSTER REIX, Christine | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | FLORIMOND, Célia | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | CAYREL, Anne | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | MAILHÉ, Amélie | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | AGNERO-RIGOT, Corentin | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | LANDREIN, Nicolas | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | DACHEUX, Denis
IDREF: 230332838 | |
| dc.contributor.author | HAVLICEK, Katharina | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | BONHIVERS, Melanie | |
| dc.contributor.author | MORRISWOOD, Brooke | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | ROBINSON, Derrick | |
| dc.date.accessioned | 2023-05-22T09:58:59Z | |
| dc.date.available | 2023-05-22T09:58:59Z | |
| dc.date.issued | 2021-11-11 | |
| dc.identifier.issn | 2076-2607 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/182218 | |
| dc.description.abstractEn | Background: In most trypanosomes, endo and exocytosis only occur at a unique organelle called the flagellar pocket (FP) and the flagellum exits the cell via the FP. Investigations of essential cytoskeleton-associated structures located at this site have revealed a number of essential proteins. The protein TbBILBO1 is located at the neck of the FP in a structure called the flagellar pocket collar (FPC) and is essential for biogenesis of the FPC and parasite survival. TbMORN1 is a protein that is present on a closely linked structure called the hook complex (HC) and is located anterior to and overlapping the collar. TbMORN1 is essential in the bloodstream form of T. brucei. We now describe the location and function of BHALIN, an essential, new FPC-HC protein. Methodology/Principal Findings: Here, we show that a newly characterised protein, BHALIN (BILBO1 Hook Associated LINker protein), is localised to both the FPC and HC and has a TbBILBO1 binding domain, which was confirmed in vitro. Knockdown of BHALIN by RNAi in the bloodstream form parasites led to cell death, indicating an essential role in cell viability. Conclusions/Significance: Our results demonstrate the essential role of a newly characterised hook complex protein, BHALIN, that influences flagellar pocket organisation and function in bloodstream form T. brucei parasites. | |
| dc.description.sponsorship | Pourquoi et comment les trypanosomes construisent un Collier de la Poche Flagellaire - ANR-20-CE91-0003 | en_US |
| dc.description.sponsorship | Alliance française contre les maladies parasitaires - ANR-11-LABX-0024 | en_US |
| dc.language.iso | EN | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by/ | |
| dc.subject.en | cytoskeleton | |
| dc.subject.en | endocytosis | |
| dc.subject.en | flagellar pocket | |
| dc.subject.en | flagellar pocket collar | |
| dc.subject.en | hook complex | |
| dc.subject.en | protozoan | |
| dc.subject.en | trypanosoma | |
| dc.title.en | Bhalin, an essential cytoskeleton-associated protein of $Trypanosoma\ brucei$ linking TbBILBO1 of the flagellar pocket collar with the hook complex | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.3390/microorganisms9112334 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biophysique | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biologie cellulaire/Organisation et fonctions cellulaires [q-bio.SC] | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biologie cellulaire/Interactions cellulaires [q-bio.CB] | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Parasitologie | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Protistologie | en_US |
| bordeaux.journal | Microorganisms | en_US |
| bordeaux.page | 2334 | en_US |
| bordeaux.volume | 9 | en_US |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.issue | 11 | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | hal | |
| hal.identifier | hal-03475266 | |
| hal.version | 1 | |
| hal.export | false | |
| workflow.import.source | hal | |
| dc.rights.cc | CC BY | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Microorganisms&rft.date=2021-11-11&rft.volume=9&rft.issue=11&rft.spage=2334&rft.epage=2334&rft.eissn=2076-2607&rft.issn=2076-2607&rft.au=BROSTER%20REIX,%20Christine&FLORIMOND,%20C%C3%A9lia&CAYREL,%20Anne&MAILH%C3%89,%20Am%C3%A9lie&AGNERO-RIGOT,%20Corentin&rft.genre=article |
