TARIS, Marion; CIACCAFAVA, Alexandre; LOJOU, Elisabeth; CASTANO, Sabine; LECOMTE, Sophie

doi:10.1016/j.vibspec.2022.103457

Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

CIACCAFAVA, Alexandre

Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

LOJOU, Elisabeth

Langue

Article de revue

Ce document a été publié dans

Vibrational Spectroscopy. 2022-11-01, vol. 123

Résumé en anglais

The functionalization of germanium by SAMs is commonly used to passivate the surface and thus prevent its oxidation. This functionalization can also be very useful to study specific interactions for instance in biosensors using protein or enzyme recognition. Several functionalization methods exist but no method has up to now been described to regenerate the Ge surface. A procedure for regenerating the surface of Ge after functionalization by SAMs is proposed in this article. The characterization of the functionalized and regenerated Ge surface by contact angle, XPS and ATR-FTIR, allows to establish that the protocol is robust. Hydrophilic SAMs (HS-(CH2)n-COOH, HS-(CH2)n-OH) and hydrophobic SAMs (HS-(CH2)n-CH3) of different chain lengths (n = 5 or 10) were tested. After ten cycles of functionalization and regeneration, the surface of germanium presents the same wettability, with a small decrease in its oxidation state. As an example of interest, this method allows to study the structure of a protein adsorbed on biomimetic surfaces (SAM type) by ATR-FTIR with a reusable commercial Ge crystal following the elimination of SAMs with a process retaining the native properties of the Ge.< Réduire

Mots clés en anglais

Reversible grafting on germanium

SAM

Protein

ATR-FTIR

XPS

URI

https://oskar-bordeaux.fr/handle/20.500.12278/172838

DOI

http://dx.doi.org/10.1016/j.vibspec.2022.103457

Project ANR

Bases moléculaires de l'immobilisation fonctionnelle d'enzymes pour des biopiles performantes - ANR-16-CE05-0024

Unités de recherche

CBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248

Voir/Ouvrir

Métadonnées

Partager cette publication !

Licence d’utilisation du document

Reversible functionalization of germanium by thiol monolayers to probe protein / surface interactions by ATR-FTIR