PASSIEUX, Renaud; SUDRE, Guillaume; MONTEMBAULT, Alexandra; RENARD, Martine; HAGEGE, Agnes; ALCOUFFE, Pierre; HADDANE, Ali; VANDESTEENE, Marie; BOUCARD, Nadege; BORDENAVE, Laurence; DAVID, Laurent

doi:10.1021/acsbiomaterials.2c00079

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PASSIEUX, Renaud
Bioingénierie tissulaire [BIOTIS]

SUDRE, Guillaume

MONTEMBAULT, Alexandra

Langue

Article de revue

Ce document a été publié dans

Acs Biomaterials Science & Engineering. 2022-04-11, vol. 8, n° 4, p. 1735-1748

Résumé en anglais

The wet spinning of cytocompatible, bioresorbable, and knittable chitosan (CTS) monofilaments would be advantageous for a variety of surgical applications. The complexation capacity of chitosan with Cu2+ or Zn2+ can be leveraged to enhance its antibacterial activity, but not at the expense of cytocompatibility. In this work, a wet-spinning process was adapted for the in situ incorporation of Cu2+ or Zn2+ with chitosan dopes to produce monofilaments at different drawing ratios (τtot) with various cation/glucosamine molar ratios, evaluated in the fibers (rCu,f and rZn,f). Cytocompatibility and antibacterial activity of wet-spun monofilaments were, respectively, quantified by in vitro live-dead assays on balb 3T3 and by different evaluations of the proliferation inhibition of Staphylococcus epidermidis (Gram+) and Escherichia coli (Gram−). Knittability was tested by a specific tensile test using a knitting needle and evaluated with an industrial knitting machine. It was found that rCu,f = 0.01 and rZn,f = 0.03 significantly increase the antibacterial activity without compromising cytocompatibility. Wet spinning with τtot = 1.6 allowed the production of knittable CTS-Cu monofilaments, as confirmed by knitting assays under industrial conditions.< Réduire

Mots clés en anglais

chitosan

wet-spinning

knittability

complexation

antibacterial

cytocompatibility

URI

https://oskar-bordeaux.fr/handle/20.500.12278/172664

DOI

http://dx.doi.org/10.1021/acsbiomaterials.2c00079

Project ANR

Lignes synchrotron françaises à l'ESRF - ANR-11-EQPX-0010

Unités de recherche

Bioingénierie Tissulaire (BioTis) - U1026