Long‐chain (LC) n‐3 polyunsaturated fatty acids (PUFAs) have drawn attention in the field of neuropsychiatric disorders, in particular depression. However, whether dietary supplementation with LC n‐3 PUFA protects from the development of mood disorders is still a matter of de-bate. In the present study, we studied the effect of a two‐month exposure to isocaloric diets containing n‐3 PUFAs in the form of relatively short‐chain (SC) (6% of rapeseed oil, enriched in α‐linolenic acid (ALA)) or LC (6% of tuna oil, enriched in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) PUFAs on behavior and synaptic plasticity of mice submitted or not to a chronic social defeat stress (CSDS), previously reported to alter emotional and social behavior, as well as synaptic plasticity in the nucleus accumbens (NAc). First, fatty acid content and lipid metabolism gene expression were measured in the NAc of mice fed a SC (control) or LC n‐3 (supplemented) PUFA diet. Our results indicate that LC n‐3 supplementation significantly increased some n‐3 PUFAs, while decreasing some n‐6 PUFAs. Then, in another cohort, control and n‐3 PUFA‐supplemented mice were subjected to CSDS, and social and emotional behaviors were assessed, together with long‐term depression plasticity in accumbal medium spiny neurons. Overall, mice fed with n‐3 PUFA supple-mentation displayed an emotional behavior profile and electrophysiological properties of medium spiny neurons which was distinct from the ones displayed by mice fed with the control diet, and this, independently of CSDS. Using the social interaction index to discriminate resilient and suscep-tible mice in the CSDS groups, n‐3 supplementation promoted resiliency. Altogether, our results pinpoint that exposure to a diet rich in LC n‐3 PUFA, as compared to a diet rich in SC n‐3 PUFA, influences the NAc fatty acid profile. In addition, electrophysiological properties and emotional behavior were altered in LC n‐3 PUFA mice, independently of CSDS. Our results bring new insights about the effect of LC n‐3 PUFA on emotional behavior and synaptic plasticity. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.< Leer menos