PAGET BLANC, Vincent; PFEFFER, Marlene; PRONOT, Marie; LAPIOS, Paul; ANGELO, Maria Florencia; WALLE, Roman; CORDELIERES, Fabrice; LEVET, Florian; CLAVEROL, Stephane; LACOMME, Sabrina; PETREL, Melina; MARTIN, Christelle; PITARD, Vincent; DE SMEDT PEYRUSSE, Veronique; BIEDERER, Thomas; PERRAIS, David; TRIFILIEFF, Pierre; HERZOG, Etienne

doi:10.1038/s41467-022-30776-9

PAGET BLANC, Vincent
Interdisciplinary Institute for Neuroscience / Institut interdisciplinaire de neurosciences [Bordeaux] [IINS]

PFEFFER, Marlene

Interdisciplinary Institute for Neuroscience / Institut interdisciplinaire de neurosciences [Bordeaux] [IINS]

PRONOT, Marie
Interdisciplinary Institute for Neuroscience / Institut interdisciplinaire de neurosciences [Bordeaux] [IINS]

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Article de revue

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Nature Communications. 2022-06-03, vol. 13, n° 1

Resumen en inglés

Dopamine transmission is involved in reward processing and motor control, and its impairment plays a central role in numerous neurological disorders. Despite its strong pathophysiological relevance, the molecular and structural organization of the dopaminergic synapse remains to be established. Here, we used targeted labelling and fluorescence activated sorting to purify striatal dopaminergic synaptosomes. We provide the proteome of dopaminergic synapses with 57 proteins specifically enriched. Beyond canonical markers of dopamine neurotransmission such as dopamine biosynthetic enzymes and cognate receptors, we validated 6 proteins not previously described as enriched. Moreover, our data reveal the adhesion of dopaminergic synapses to glutamatergic, GABAergic or cholinergic synapses in structures we named “dopamine hub synapses”. At glutamatergic synapses, pre- and postsynaptic markers are significantly increased upon association with dopamine synapses. Dopamine hub synapses may thus support local dopaminergic signalling, complementing volume transmission thought to be the major mechanism by which monoamines modulate network activity. © 2022, The Author(s).< Leer menos

URI

https://oskar-bordeaux.fr/handle/20.500.12278/171574

DOI

http://dx.doi.org/10.1038/s41467-022-30776-9

Proyecto ANR

Initiative d'excellence de l'Université de Bordeaux - ANR-10-IDEX-0003
Bordeaux Region Aquitaine Initiative for Neuroscience - ANR-10-LABX-0043
Développment d'une infrastructure française distribuée coordonnée - ANR-10-INBS-0004
Impact de la composition lipidique membranaire sur la transmission dopaminergique dépendante du récepteur D2 et la motivation

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NutriNeuro (Laboratoire de Nutrition et Neurobiologie Intégrée) - UMR 1286

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A synaptomic analysis reveals dopamine hub synapses in the mouse striatum

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DOI

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Centros de investigación