Viral Double-Stranded RNAs (dsRNAs) from Plants: Alternative Nucleic Acid Substrates for High-Throughput Sequencing.
Langue
en
Chapitre d'ouvrage
Ce document a été publié dans
Viral Metagenomics: Methods and Protocols, Viral Metagenomics: Methods and Protocols. 2018, vol. 1746
Springer Science + Business Media LLC
Résumé en anglais
High-throughput sequencing (or next-generation sequencing-NGS) is an emerging technology that allows the detection of plant viruses without any prior knowledge. Various sequencing techniques and various templates can be ...Lire la suite >
High-throughput sequencing (or next-generation sequencing-NGS) is an emerging technology that allows the detection of plant viruses without any prior knowledge. Various sequencing techniques and various templates can be used as substrate for NGS. This chapter describes an optimized protocol for the extraction of double-stranded RNAs (dsRNAs) from a wide range of plants and for their random amplification prior to NGS sequencing.< Réduire
Mots clés
Metagenomics
Mots clés en anglais
Diagnostic
Double-stranded (ds) RNA
High-throughput sequencing
NGS
Virome
Origine
Importé de halUnités de recherche