Viral Double-Stranded RNAs (dsRNAs) from Plants: Alternative Nucleic Acid Substrates for High-Throughput Sequencing.
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | MARAIS, Armelle | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | FAURE, Chantal | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | BERGEY, Bernard | |
| hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
| dc.contributor.author | CANDRESSE, Thierry | |
| dc.date.issued | 2018 | |
| dc.identifier.isbn | 978-1-4939-7683-6_4 | |
| dc.description.abstractEn | High-throughput sequencing (or next-generation sequencing-NGS) is an emerging technology that allows the detection of plant viruses without any prior knowledge. Various sequencing techniques and various templates can be used as substrate for NGS. This chapter describes an optimized protocol for the extraction of double-stranded RNAs (dsRNAs) from a wide range of plants and for their random amplification prior to NGS sequencing. | |
| dc.language.iso | en | |
| dc.publisher | Springer Science + Business Media LLC | |
| dc.publisher.location | (united states) | |
| dc.source.title | Viral Metagenomics: Methods and Protocols | |
| dc.subject | Metagenomics | |
| dc.subject.en | Diagnostic | |
| dc.subject.en | Double-stranded (ds) RNA | |
| dc.subject.en | High-throughput sequencing | |
| dc.subject.en | NGS | |
| dc.subject.en | Virome | |
| dc.title.en | Viral Double-Stranded RNAs (dsRNAs) from Plants: Alternative Nucleic Acid Substrates for High-Throughput Sequencing. | |
| dc.type | Chapitre d'ouvrage | |
| dc.identifier.doi | 10.1007/978-1-4939-7683-6_4 | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie | |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie | |
| bordeaux.volume | 1746 | |
| bordeaux.title.proceeding | Viral Metagenomics: Methods and Protocols | |
| hal.identifier | hal-02789974 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-02789974v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.btitle=Viral%20Metagenomics:%20Methods%20and%20Protocols&rft.date=2018&rft.volume=1746&rft.au=MARAIS,%20Armelle&FAURE,%20Chantal&BERGEY,%20Bernard&CANDRESSE,%20Thierry&rft.isbn=978-1-4939-7683-6_4&rft.genre=unknown |
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