Viral Double-Stranded RNAs (dsRNAs) from Plants: Alternative Nucleic Acid Substrates for High-Throughput Sequencing.
Idioma
en
Chapitre d'ouvrage
Este ítem está publicado en
Viral Metagenomics: Methods and Protocols, Viral Metagenomics: Methods and Protocols. 2018, vol. 1746
Springer Science + Business Media LLC
Resumen en inglés
High-throughput sequencing (or next-generation sequencing-NGS) is an emerging technology that allows the detection of plant viruses without any prior knowledge. Various sequencing techniques and various templates can be ...Leer más >
High-throughput sequencing (or next-generation sequencing-NGS) is an emerging technology that allows the detection of plant viruses without any prior knowledge. Various sequencing techniques and various templates can be used as substrate for NGS. This chapter describes an optimized protocol for the extraction of double-stranded RNAs (dsRNAs) from a wide range of plants and for their random amplification prior to NGS sequencing.< Leer menos
Palabras clave
Metagenomics
Palabras clave en inglés
Diagnostic
Double-stranded (ds) RNA
High-throughput sequencing
NGS
Virome
Orígen
Importado de HalCentros de investigación