Viral Double-Stranded RNAs (dsRNAs) from Plants: Alternative Nucleic Acid Substrates for High-Throughput Sequencing.
Language
en
Chapitre d'ouvrage
This item was published in
Viral Metagenomics: Methods and Protocols, Viral Metagenomics: Methods and Protocols. 2018, vol. 1746
Springer Science + Business Media LLC
English Abstract
High-throughput sequencing (or next-generation sequencing-NGS) is an emerging technology that allows the detection of plant viruses without any prior knowledge. Various sequencing techniques and various templates can be ...Read more >
High-throughput sequencing (or next-generation sequencing-NGS) is an emerging technology that allows the detection of plant viruses without any prior knowledge. Various sequencing techniques and various templates can be used as substrate for NGS. This chapter describes an optimized protocol for the extraction of double-stranded RNAs (dsRNAs) from a wide range of plants and for their random amplification prior to NGS sequencing.Read less <
Keywords
Metagenomics
English Keywords
Diagnostic
Double-stranded (ds) RNA
High-throughput sequencing
NGS
Virome
Origin
Hal importedCollections