Mapping the phytoplasma chromosome
Language
en
Chapitre d'ouvrage
This item was published in
Phytoplasma: Methods and Protocols, Phytoplasma: Methods and Protocols. 2013, vol. 938
Springer Science+Business Media
English Abstract
Physical and genetic mapping of the phytoplasma chromosome can be a useful tool in a genome sequencing project in order to assemble the in silico-predicted contigs robustly. Mapping consists of four distinct steps: preparation ...Read more >
Physical and genetic mapping of the phytoplasma chromosome can be a useful tool in a genome sequencing project in order to assemble the in silico-predicted contigs robustly. Mapping consists of four distinct steps: preparation of phytoplasma chromosomes from infected plants, single- and double-digestion of chromosomes with rare-cutting restriction enzymes, separation of large DNA fragments by pulsed-field gel electrophoresis, and hybridization with various genetic markers. Materials and methods needed for each step are described and the technique is illustrated using the flavescence doree phytoplasma genome map as an example.Read less <
Keywords
flavescence dorée
mollicutes
English Keywords
Chromosome Mapping/ methods
phytoplasma
genetic map
physical map
pulsed-field gel electrophoresis
restriction endonucleases
southern blot
Origin
Hal importedCollections