MALEMBIC-MAHER, Sylvie; CARLE, Patricia

doi:10.1007/978-1-62703-089-2_34

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MALEMBIC-MAHER, Sylvie
Biologie du fruit et pathologie [BFP]

CARLE, Patricia
Biologie du fruit et pathologie [BFP]

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Este ítem está publicado en

Phytoplasma: Methods and Protocols, Phytoplasma: Methods and Protocols. 2013, vol. 938

Springer Science+Business Media

Resumen en inglés

Physical and genetic mapping of the phytoplasma chromosome can be a useful tool in a genome sequencing project in order to assemble the in silico-predicted contigs robustly. Mapping consists of four distinct steps: preparation of phytoplasma chromosomes from infected plants, single- and double-digestion of chromosomes with rare-cutting restriction enzymes, separation of large DNA fragments by pulsed-field gel electrophoresis, and hybridization with various genetic markers. Materials and methods needed for each step are described and the technique is illustrated using the flavescence doree phytoplasma genome map as an example.< Leer menos

Palabras clave

flavescence dorée

mollicutes

Palabras clave en inglés

Chromosome Mapping/ methods

phytoplasma

genetic map

physical map

pulsed-field gel electrophoresis

restriction endonucleases

southern blot