Photothermal Absorption Correlation Spectroscopy
Language
en
Article de revue
This item was published in
ACS Nano. 2009-02-24, vol. 3, n° 2, p. 345-350
American Chemical Society
English Abstract
Fluorescence correlation spectroscopy (FCS) is a popular technique, complementary to cell imaging for the investigation of dynamic processes in living cells. Based on fluorescence, this single molecule method suffers from ...Read more >
Fluorescence correlation spectroscopy (FCS) is a popular technique, complementary to cell imaging for the investigation of dynamic processes in living cells. Based on fluorescence, this single molecule method suffers from artifacts originating from the poor fluorophore photophysics: photobleaching, blinking, and saturation. To circumvent these limitations we present here a new correlation method called photothermal absorption correlation spectroscopy (PhACS) which relies on the absorption properties of tiny nano-objects. PhACS is based on the photothermal heterodyne detection technique and measures akin FCS, the time correlation function of the detected signals. Application of this technique to the precise determination of the hydrodynamic sizes of different functionalized gold nanoparticles are presented, highlighting the potential of this method.Read less <
English Keywords
photothermal detection
correlation spectroscopy
single molecule
gold nanoparticles
Origin
Hal imported