GUICHOUX, Erwan; LAGACHE, Lelia; WAGNER, Stefanie; LÉGER, Patrick; PETIT, Remy

doi:10.1111/j.1755-0998.2011.02983.x

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GUICHOUX, Erwan
Biodiversité, Gènes & Communautés [BioGeCo]
Centre de recherche Pernod Ricard

LAGACHE, Lelia
Biodiversité, Gènes & Communautés [BioGeCo]

WAGNER, Stefanie
Biodiversité, Gènes & Communautés [BioGeCo]
Steinmann Institut

Langue

Article de revue

Ce document a été publié dans

Molecular Ecology Resources. 2011, vol. 11, n° 3, p. 578-585

Wiley/Blackwell

Résumé en anglais

Multiplex PCR is a fast and cost-effective technique allowing increased genotyping throughput of microsatellites. We developed two multiplexes for Quercus petraea and Q. robur, a 12-plex of EST-SSRs (eSSRs) and an 8-plex of genomic SSRs (gSSRs). We studied the origin of allele calling errors at the human reader and software levels. We showed that the robustness of allele identification can be improved by binning on raw peak sizes prior to genetic data analysis. We checked through simulation the power of these markers for species delimitation and hybrid detection. The resolution achieved with all 20 markers was greatly improved compared to that of previous studies based on a subset of the markers. Preliminary PCR tests suggest that these multiplexes might be useful to study other oak species as well. The strategy used for multiplex microsatellite development (from PCR conditions to the definition of allele calling rules) should be broadly applicable.< Réduire

Mots clés

QUERCUS ROBUR

QUERCUS PETRAEA

QUERCUS SPP.

MICROSATELLITES

QUERCUS SESSILIFLORA

CHENE ROUVRE

Mots clés en anglais

MANUAL BINNING

MOLECULAR IDENTIFICATION

CHENE

CHENE PEDONCULE

MULTIPLEXE

ISOLATION DE L'ADN

DOI

http://dx.doi.org/10.1111/j.1755-0998.2011.02983.x

Project ANR

Relations entre diversité génétique et réponse écologique aux changements environementaux : les arbres forestiers comme modèles d'étude

Origine

Importé de hal

Unités de recherche

BioGeCo (Biodiversité Gènes & Communautés) - UMR 1202