Two highly validated multiplexes (12-plex and 8-plex) for species delimitation and parentage analysis in oaks (Quercus spp.)
Language
en
Article de revue
This item was published in
Molecular Ecology Resources. 2011, vol. 11, n° 3, p. 578-585
Wiley/Blackwell
English Abstract
Multiplex PCR is a fast and cost-effective technique allowing increased genotyping throughput of microsatellites. We developed two multiplexes for Quercus petraea and Q. robur, a 12-plex of EST-SSRs (eSSRs) and an 8-plex ...Read more >
Multiplex PCR is a fast and cost-effective technique allowing increased genotyping throughput of microsatellites. We developed two multiplexes for Quercus petraea and Q. robur, a 12-plex of EST-SSRs (eSSRs) and an 8-plex of genomic SSRs (gSSRs). We studied the origin of allele calling errors at the human reader and software levels. We showed that the robustness of allele identification can be improved by binning on raw peak sizes prior to genetic data analysis. We checked through simulation the power of these markers for species delimitation and hybrid detection. The resolution achieved with all 20 markers was greatly improved compared to that of previous studies based on a subset of the markers. Preliminary PCR tests suggest that these multiplexes might be useful to study other oak species as well. The strategy used for multiplex microsatellite development (from PCR conditions to the definition of allele calling rules) should be broadly applicable.Read less <
Keywords
QUERCUS ROBUR
QUERCUS PETRAEA
QUERCUS SPP.
MICROSATELLITES
QUERCUS SESSILIFLORA
CHENE ROUVRE
English Keywords
MANUAL BINNING
MOLECULAR IDENTIFICATION
CHENE
CHENE PEDONCULE
MULTIPLEXE
ISOLATION DE L'ADN
ANR Project
Relations entre diversité génétique et réponse écologique aux changements environementaux : les arbres forestiers comme modèles d'étude
Origin
Hal imported