Two highly validated multiplexes (12-plex and 8-plex) for species delimitation and parentage analysis in oaks (Quercus spp.)
| hal.structure.identifier | Biodiversité, Gènes & Communautés [BioGeCo] | |
| hal.structure.identifier | Centre de recherche Pernod Ricard | |
| dc.contributor.author | GUICHOUX, Erwan | |
| hal.structure.identifier | Biodiversité, Gènes & Communautés [BioGeCo] | |
| dc.contributor.author | LAGACHE, Lelia | |
| hal.structure.identifier | Biodiversité, Gènes & Communautés [BioGeCo] | |
| hal.structure.identifier | Steinmann Institut | |
| dc.contributor.author | WAGNER, Stefanie | |
| hal.structure.identifier | Biodiversité, Gènes & Communautés [BioGeCo] | |
| dc.contributor.author | LÉGER, Patrick | |
| hal.structure.identifier | Biodiversité, Gènes & Communautés [BioGeCo] | |
| dc.contributor.author | PETIT, Remy | |
| dc.date.issued | 2011 | |
| dc.identifier.issn | 1755-098X | |
| dc.description.abstractEn | Multiplex PCR is a fast and cost-effective technique allowing increased genotyping throughput of microsatellites. We developed two multiplexes for Quercus petraea and Q. robur, a 12-plex of EST-SSRs (eSSRs) and an 8-plex of genomic SSRs (gSSRs). We studied the origin of allele calling errors at the human reader and software levels. We showed that the robustness of allele identification can be improved by binning on raw peak sizes prior to genetic data analysis. We checked through simulation the power of these markers for species delimitation and hybrid detection. The resolution achieved with all 20 markers was greatly improved compared to that of previous studies based on a subset of the markers. Preliminary PCR tests suggest that these multiplexes might be useful to study other oak species as well. The strategy used for multiplex microsatellite development (from PCR conditions to the definition of allele calling rules) should be broadly applicable. | |
| dc.description.sponsorship | Relations entre diversité génétique et réponse écologique aux changements environementaux : les arbres forestiers comme modèles d'étude | |
| dc.language.iso | en | |
| dc.publisher | Wiley/Blackwell | |
| dc.subject | QUERCUS ROBUR | |
| dc.subject | QUERCUS PETRAEA | |
| dc.subject | QUERCUS SPP. | |
| dc.subject | MICROSATELLITES | |
| dc.subject | QUERCUS SESSILIFLORA | |
| dc.subject | CHENE ROUVRE | |
| dc.subject.en | MANUAL BINNING | |
| dc.subject.en | MOLECULAR IDENTIFICATION | |
| dc.subject.en | CHENE | |
| dc.subject.en | CHENE PEDONCULE | |
| dc.subject.en | MULTIPLEXE | |
| dc.subject.en | ISOLATION DE L'ADN | |
| dc.title.en | Two highly validated multiplexes (12-plex and 8-plex) for species delimitation and parentage analysis in oaks (Quercus spp.) | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1111/j.1755-0998.2011.02983.x | |
| dc.subject.hal | Sciences du Vivant [q-bio] | |
| bordeaux.journal | Molecular Ecology Resources | |
| bordeaux.page | 578-585 | |
| bordeaux.volume | 11 | |
| bordeaux.issue | 3 | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-02646724 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-02646724v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Molecular%20Ecology%20Resources&rft.date=2011&rft.volume=11&rft.issue=3&rft.spage=578-585&rft.epage=578-585&rft.eissn=1755-098X&rft.issn=1755-098X&rft.au=GUICHOUX,%20Erwan&LAGACHE,%20Lelia&WAGNER,%20Stefanie&L%C3%89GER,%20Patrick&PETIT,%20Remy&rft.genre=article |
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