Current trends in microsatellite genotyping
LEPAIS, Olivier
Biodiversité, Gènes & Communautés [BioGeCo]
School of Biological and Environmental Sciences
< Reduce
Biodiversité, Gènes & Communautés [BioGeCo]
School of Biological and Environmental Sciences
Language
en
Article de revue
This item was published in
Molecular Ecology Resources. 2011, vol. 11, n° 4, p. 591-611
Wiley/Blackwell
English Abstract
Microsatellites have been popular molecular markers ever since their advent in the late eighties. Despite growing competition from new genotyping and sequencing techniques, the use of these versatile and cost-effective ...Read more >
Microsatellites have been popular molecular markers ever since their advent in the late eighties. Despite growing competition from new genotyping and sequencing techniques, the use of these versatile and cost-effective markers continues to increase, boosted by successive technical advances. First, methods for multiplexing PCR have considerably improved over the last years, thereby decreasing genotyping costs and increasing throughput. Second, next-generation sequencing technologies allow the identification of large numbers of microsatellite loci at reduced cost in non-model species. As a consequence, more stringent selection of loci is possible, thereby further enhancing multiplex quality and efficiency. However, current practices are lagging behind. By surveying recently published population genetic studies relying on simple sequence repeats, we show that more than half of the studies lack appropriate quality controls and do not make use of multiplex PCR. To make the most of the latest technical developments, we outline the need for a well-established strategy including standardized high-throughput bench protocols and specific bioinformatic tools, from primer design to allele calling.Read less <
Keywords
GENETIQUE DES POPULATIONS
CARTOGRAPHIE GENETIQUE
CONTRÔLE DE QUALITÉ
English Keywords
BINNING
HIGH THROUGHPUT
NEXTGEN SEQUENCING
MULTIPLEXING
QUALITY CONTROL
SSR
ANR Project
Relations entre diversité génétique et réponse écologique aux changements environementaux : les arbres forestiers comme modèles d'étude
Origin
Hal imported