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Current trends in microsatellite genotyping

hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
hal.structure.identifierCentre de recherche Pernod Ricard
dc.contributor.authorGUICHOUX, Erwan
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLAGACHE, Lelia
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
hal.structure.identifierSteinmann Institut
dc.contributor.authorWAGNER, Stefanie
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorCHAUMEIL, Philippe
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLÉGER, Patrick
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
hal.structure.identifierSchool of Biological and Environmental Sciences
dc.contributor.authorLEPAIS, Olivier
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLEPOITTEVIN, Camille
hal.structure.identifierInteractions Biotiques et Santé Végétale [IBSV]
dc.contributor.authorMALAUSA, Thibaut
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorREVARDEL, Emmanuelle
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorSALIN, Franck
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorPETIT, Remy
dc.date.issued2011
dc.identifier.issn1755-098X
dc.description.abstractEnMicrosatellites have been popular molecular markers ever since their advent in the late eighties. Despite growing competition from new genotyping and sequencing techniques, the use of these versatile and cost-effective markers continues to increase, boosted by successive technical advances. First, methods for multiplexing PCR have considerably improved over the last years, thereby decreasing genotyping costs and increasing throughput. Second, next-generation sequencing technologies allow the identification of large numbers of microsatellite loci at reduced cost in non-model species. As a consequence, more stringent selection of loci is possible, thereby further enhancing multiplex quality and efficiency. However, current practices are lagging behind. By surveying recently published population genetic studies relying on simple sequence repeats, we show that more than half of the studies lack appropriate quality controls and do not make use of multiplex PCR. To make the most of the latest technical developments, we outline the need for a well-established strategy including standardized high-throughput bench protocols and specific bioinformatic tools, from primer design to allele calling.
dc.description.sponsorshipRelations entre diversité génétique et réponse écologique aux changements environementaux : les arbres forestiers comme modèles d'étude - ANR-08-BDVA-0006
dc.language.isoen
dc.publisherWiley/Blackwell
dc.subjectGENETIQUE DES POPULATIONS
dc.subjectCARTOGRAPHIE GENETIQUE
dc.subjectCONTRÔLE DE QUALITÉ
dc.subject.enBINNING
dc.subject.enHIGH THROUGHPUT
dc.subject.enNEXTGEN SEQUENCING
dc.subject.enMULTIPLEXING
dc.subject.enQUALITY CONTROL
dc.subject.enSSR
dc.title.enCurrent trends in microsatellite genotyping
dc.typeArticle de revue
dc.identifier.doi10.1111/j.1755-0998.2011.03014.x
dc.subject.halSciences du Vivant [q-bio]/Ecologie, Environnement
bordeaux.journalMolecular Ecology Resources
bordeaux.page591-611
bordeaux.volume11
bordeaux.issue4
bordeaux.peerReviewedoui
hal.identifierhal-02652759
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02652759v1
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