GUICHOUX, Erwan; LAGACHE, Lelia; WAGNER, Stefanie; CHAUMEIL, Philippe; LÉGER, Patrick; LEPAIS, Olivier; LEPOITTEVIN, Camille; MALAUSA, Thibaut; REVARDEL, Emmanuelle; SALIN, Franck; PETIT, Remy

doi:10.1111/j.1755-0998.2011.03014.x

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GUICHOUX, Erwan
Biodiversité, Gènes & Communautés [BioGeCo]
Centre de recherche Pernod Ricard

LAGACHE, Lelia
Biodiversité, Gènes & Communautés [BioGeCo]

WAGNER, Stefanie
Biodiversité, Gènes & Communautés [BioGeCo]
Steinmann Institut

Langue

Article de revue

Ce document a été publié dans

Molecular Ecology Resources. 2011, vol. 11, n° 4, p. 591-611

Wiley/Blackwell

Résumé en anglais

Microsatellites have been popular molecular markers ever since their advent in the late eighties. Despite growing competition from new genotyping and sequencing techniques, the use of these versatile and cost-effective markers continues to increase, boosted by successive technical advances. First, methods for multiplexing PCR have considerably improved over the last years, thereby decreasing genotyping costs and increasing throughput. Second, next-generation sequencing technologies allow the identification of large numbers of microsatellite loci at reduced cost in non-model species. As a consequence, more stringent selection of loci is possible, thereby further enhancing multiplex quality and efficiency. However, current practices are lagging behind. By surveying recently published population genetic studies relying on simple sequence repeats, we show that more than half of the studies lack appropriate quality controls and do not make use of multiplex PCR. To make the most of the latest technical developments, we outline the need for a well-established strategy including standardized high-throughput bench protocols and specific bioinformatic tools, from primer design to allele calling.< Réduire

Mots clés

GENETIQUE DES POPULATIONS

CARTOGRAPHIE GENETIQUE

CONTRÔLE DE QUALITÉ

Mots clés en anglais

BINNING

HIGH THROUGHPUT

NEXTGEN SEQUENCING

MULTIPLEXING

QUALITY CONTROL

SSR

DOI

http://dx.doi.org/10.1111/j.1755-0998.2011.03014.x

Project ANR

Relations entre diversité génétique et réponse écologique aux changements environementaux : les arbres forestiers comme modèles d'étude - ANR-08-BDVA-0006

Origine

Importé de hal

Unités de recherche

BioGeCo (Biodiversité Gènes & Communautés) - UMR 1202