Development of EST-SSR markers from an inner bark cDNA library of Fagus crenata (Fagaceae)
UENO, Saneyoshi
Biodiversité, Gènes & Communautés [BioGeCo]
Forestry and Forest Products Research Institute [FFPRI]
Centre de Coopération Internationale en Recherche Agronomique pour le Développement [Cirad]
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Biodiversité, Gènes & Communautés [BioGeCo]
Forestry and Forest Products Research Institute [FFPRI]
Centre de Coopération Internationale en Recherche Agronomique pour le Développement [Cirad]
UENO, Saneyoshi
Biodiversité, Gènes & Communautés [BioGeCo]
Forestry and Forest Products Research Institute [FFPRI]
Centre de Coopération Internationale en Recherche Agronomique pour le Développement [Cirad]
< Reduce
Biodiversité, Gènes & Communautés [BioGeCo]
Forestry and Forest Products Research Institute [FFPRI]
Centre de Coopération Internationale en Recherche Agronomique pour le Développement [Cirad]
Language
en
Article de revue
This item was published in
Conservation Genetics. 2009, vol. 10, n° 5, p. 1477-1485
Springer Verlag
English Abstract
Fagus crenata Blume is widely distributed throughout Japanese cool-temperate deciduous broad-leaved forests, but there are two divergent groups of populations in areas with contrasting winter climates separated by Japan’s ...Read more >
Fagus crenata Blume is widely distributed throughout Japanese cool-temperate deciduous broad-leaved forests, but there are two divergent groups of populations in areas with contrasting winter climates separated by Japan’s Central Mountain Range. To facilitate investigations of adaptive genetic differentiation of the species using potentially functional genes, we have collected Expressed Sequence Tags and developed Simple Sequence Repeat markers using a cDNA library constructed from cambium and surrounding tissues. In total, 270 primer pairs were designed, and 87 of the corresponding loci showed polymorphism in 16 individuals, with 2–21 alleles per locus and expected heterozygosities ranging from 0.06 to 0.97. EST-SSR markers developed in the present study will be useful for genomic analyses of F. crenata populations.Read less <
Keywords
QUERCUS
MICROSATELLITE
ADAPTATION GENETIQUE
English Keywords
FAGUS CRENATA
ADAPTATION
EXPRESSED SEQUENCE TAG
JAPANESE BEECH
CHENE
HETRE
Origin
Hal imported