UENO, Saneyoshi; TAGUCHI, Yuriko; TOMARU, Nobuhiro; TSUMURA, Yoshihiko

doi:10.1007/s10592-008-9764-1

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UENO, Saneyoshi
Biodiversité, Gènes & Communautés [BioGeCo]
Forestry and Forest Products Research Institute [FFPRI]
Centre de Coopération Internationale en Recherche Agronomique pour le Développement [Cirad]

TAGUCHI, Yuriko
Forestry and Forest Products Research Institute [FFPRI]

TOMARU, Nobuhiro
Graduate School of Bioagricultural Sciences

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Article de revue

Ce document a été publié dans

Conservation Genetics. 2009, vol. 10, n° 5, p. 1477-1485

Springer Verlag

Résumé en anglais

Fagus crenata Blume is widely distributed throughout Japanese cool-temperate deciduous broad-leaved forests, but there are two divergent groups of populations in areas with contrasting winter climates separated by Japan’s Central Mountain Range. To facilitate investigations of adaptive genetic differentiation of the species using potentially functional genes, we have collected Expressed Sequence Tags and developed Simple Sequence Repeat markers using a cDNA library constructed from cambium and surrounding tissues. In total, 270 primer pairs were designed, and 87 of the corresponding loci showed polymorphism in 16 individuals, with 2–21 alleles per locus and expected heterozygosities ranging from 0.06 to 0.97. EST-SSR markers developed in the present study will be useful for genomic analyses of F. crenata populations.< Réduire

Mots clés

QUERCUS

MICROSATELLITE

ADAPTATION GENETIQUE

Mots clés en anglais

FAGUS CRENATA

ADAPTATION

EXPRESSED SEQUENCE TAG

JAPANESE BEECH

CHENE

HETRE