The stenlying effect of high hydrostatic pressure on thermally and hydrolytically labile nanosized carriers
ANDREMONT, Antoine
Physico-chimie, pharmacotechnie, biopharmacie [PCPB]
Laboratoire de Bactériologie
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Physico-chimie, pharmacotechnie, biopharmacie [PCPB]
Laboratoire de Bactériologie
Language
en
Article de revue
This item was published in
Pharmaceutical Research. 2003, vol. 20, n° 4, p. 674-683
American Association of Pharmaceutical Scientists
English Abstract
To investigate whether high hydrostatic pressure (HHP) treatment allows the sterilization of thermosensitive polymer nanoparticle suspensions without jeopardizing their physicochemical integrity. METHODS: Application of ...Read more >
To investigate whether high hydrostatic pressure (HHP) treatment allows the sterilization of thermosensitive polymer nanoparticle suspensions without jeopardizing their physicochemical integrity. METHODS: Application of HHP was explored on a wide variety of thermosensitive poly(cyanoacrylate) nanoparticles, varying by their type (nanospheres or nanocapsules), by their preparation method (nanoprecipitation or emulsion/solvent evaporation), as well as by their surface characteristics. Physicochemical characterization before and after pressurization included turbidimetry, size measurement, zeta potential, scanning electron microscopy and infrared analysis. A sterility test also conducted according to pharmacopoeial requirements on an importantly contaminated nanoparticle suspension. RESULTS: Poly(cyanoacrylate) nanoparticles appeared to be extremely baroresistant. Continuous or oscillatory HHP treatment up to 500 MPa during 30 min induced generally neither physical, nor chemical damage. However, precautions should be taken when surface modifiers are adsorbed onto nanoparticles, as a layer destabilization may occur. Finally, this process allowed the successful inactivation of vegetative bacteria, yeast, and fungi. CONCLUSIONS: This work proposes HHP as a new method for polymer drug carriers sterilization, taking into account that further exploration in this area is needed to propose novel protocols for spores inactivation.Read less <
English Keywords
High hydrostatic pressure processing
Nanoparticles
Poly(cyanoacrylate)
Sterilization
Surface modifiers
Origin
Hal imported