Detection of Short DNA Sequences with DNA Nanopores
Langue
EN
Article de revue
Ce document a été publié dans
ChemPhysChem. 2022, vol. 23, n° 7
Résumé en anglais
Several studies suggest strong correlation between different types of cancer and the relative concentration of short circulating RNA sequences (miRNA). Because of short length and low concentration, miRNA detection is not ...Lire la suite >
Several studies suggest strong correlation between different types of cancer and the relative concentration of short circulating RNA sequences (miRNA). Because of short length and low concentration, miRNA detection is not easy. Standard methods such as RT-PCR require both the standard PCR amplification step and a preliminary additional step of reverse transcription. In this paper, we investigate the use of DNA nanopores as a tool to detect short oligonucleotide sequences at the single molecule level. These nanostructures show two different conformations depending on the presence of DNA analogues of miRNA sequences. By monitoring current across a lipid bilayer, we show that this change of conformation translates to different levels of conductance.< Réduire
Mots clés en anglais
DNA origami
nanopore
self-assembly
droplet interface bilayer
microRNA
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