Phosphoinositides (PIPs) are present in very small amounts but are essential for cell signaling, morphogenesis, and polarity. By mass spectrometry, we demonstrated that some PIPs with stearic acyl chains were strongly disturbed in a psi1Δ Saccharomyces cerevisiae yeast strain deficient in the specific incorporation of a stearoyl chain at the sn-1 position of phosphatidylinositol. The absence of PIPs containing stearic acid induced disturbances in intracellular trafficking, although the total amount of PIPs was not diminished. Changes in PIPs also induced alterations in the budding pattern and defects in actin cytoskeleton organization (cables and patches). Moreover, when the PSI1 gene was impaired, a high proportion of cells with bipolar cortical actin patches that occurred concomitantly with the bipolar localization of Cdc42p was specifically found among diploid cells. This bipolar cortical actin phenotype, never previously described, was also detected in a bud9Δ/bud9Δ strain. Very interestingly, overexpression of PSI1 reversed this phenotype.< Réduire

Mots clés en anglais

Actins

Acyltransferases

Cell Polarity

Gene Deletion

Phosphatidylinositols

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

Stearic Acids

URI

https://oskar-bordeaux.fr/handle/20.500.12278/124678

DOI

http://dx.doi.org/10.1128/MCB.00843-15

Project ANR

Développement d’une infrastructure française distribuée pour la métabolomique dédiée à l’innovation - ANR-11-INBS-0010/11-INBS-0010
Développment d'une infrastructure française distribuée coordonnée - ANR-10-INBS-04-01/10-INBS-0004

Unités de recherche

Laboratoire de Biogenèse Membranaire (LBM) - UMR 5200

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Requirement of Phosphoinositides Containing Stearic Acid To Control Cell Polarity

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DOI

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