Requirement of Phosphoinositides Containing Stearic Acid To Control Cell Polarity
Language
EN
Article de revue
This item was published in
Molecular and Cellular Biology. 2016-02-16, vol. 36, n° 5, p. 765-80
English Abstract
Phosphoinositides (PIPs) are present in very small amounts but are essential for cell signaling, morphogenesis, and polarity. By mass spectrometry, we demonstrated that some PIPs with stearic acyl chains were strongly ...Read more >
Phosphoinositides (PIPs) are present in very small amounts but are essential for cell signaling, morphogenesis, and polarity. By mass spectrometry, we demonstrated that some PIPs with stearic acyl chains were strongly disturbed in a psi1Δ Saccharomyces cerevisiae yeast strain deficient in the specific incorporation of a stearoyl chain at the sn-1 position of phosphatidylinositol. The absence of PIPs containing stearic acid induced disturbances in intracellular trafficking, although the total amount of PIPs was not diminished. Changes in PIPs also induced alterations in the budding pattern and defects in actin cytoskeleton organization (cables and patches). Moreover, when the PSI1 gene was impaired, a high proportion of cells with bipolar cortical actin patches that occurred concomitantly with the bipolar localization of Cdc42p was specifically found among diploid cells. This bipolar cortical actin phenotype, never previously described, was also detected in a bud9Δ/bud9Δ strain. Very interestingly, overexpression of PSI1 reversed this phenotype.Read less <
English Keywords
Actins
Acyltransferases
Cell Polarity
Gene Deletion
Phosphatidylinositols
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Stearic Acids
ANR Project
Développement d’une infrastructure française distribuée pour la métabolomique dédiée à l’innovation - ANR-11-INBS-0010/11-INBS-0010
Développment d'une infrastructure française distribuée coordonnée - ANR-10-INBS-04-01/10-INBS-0004
Développment d'une infrastructure française distribuée coordonnée - ANR-10-INBS-04-01/10-INBS-0004