BROSSAUD, Julie; ROUMES, Helene; HELBLING, Jean Christophe; MOISAN, Marie Pierre; PALLET, Veronique; FERREIRA, Guillaume; BIYONG, Essi-Fanny; REDONNET, Anabelle; CORCUFF, Jean-Benoit

doi:10.1016/j.mcn.2017.05.001

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BROSSAUD, Julie
Nutrition et Neurobiologie intégrée [NutriNeuro]

ROUMES, Helene
Nutrition et Neurobiologie intégrée [NutriNeuro]

HELBLING, Jean Christophe
Nutrition et Neurobiologie intégrée [NutriNeuro]

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Molecular and Cellular Neuroscience. 2017, vol. 82, p. 96-104

Résumé en anglais

Glucocorticoid receptor (GR) function is modulated by phosphorylation. As retinoic acid (RA) can activate some cytoplasmic kinases able to phosphorylate GR, we investigated whether RA could modulate GR phosphorylation in neuronal cells in a context of long-term glucocorticoid exposure. A 4-day treatment of dexamethasone (Dex) plus RA, showed that RA potentiated the (Dex)-induced phosphorylation on GR Serine 220 (pSer220GR) in the nucleus of a hippocampal HT22 cell line. This treatment increased the cytoplasmic ratio of p35/p25 proteins, which are major CDK5 cofactors. Roscovitine, a pharmacological CDK5 inhibitor, or a siRNA against CDK5 prevented RA potentiation of GR phosphorylation. Furthermore, roscovitine counter-acted the effect of RA on GR sensitive target proteins such as BDNF or tissue-transglutaminase. These data help understanding the interaction between RA- and glucocorticoid-signalling pathways, both of which have strong influences on the adult brain.< Réduire

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Retinoic acid

Glucocorticoid receptor

Phosphorylation

Cyclin-dependent kinase 5

Hippocampus

Neuron

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Retinoic acid increases glucocorticoid receptor phosphorylation via cyclin-dependent kinase 5.

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