17 beta-Estradiol (17 beta-E-2) is a steroid with pleiotropic actions. In addition to being a sexual hormone, it is also produced in the brain where it modulates the reproductive axis. It has been shown that 17 beta-E-2 also acts on synaptic plasticity and plays a role in neurological pathways and in neurodegenerative diseases. Assaying this steroid in the brain is thus interesting to improve our knowledge of 17 beta-E-2 effects in the brain. However, 17 beta-E-2 concentration in the central nervous system has been reported to be of a few nanograms per gram wet weight (nanomolar range concentration); therefore, its quantification requires both an efficient extraction process and a sensitive detection method. Herein is presented a derivatization-free procedure based on solid-phase extraction followed by LC-MS/MS analysis, targeted on 17 beta-E-2, its isomer17 alpha-E-2, and its metabolites estrone (E-1) and estriol (E-3). This extraction process allowed reaching 96% 17 beta-E-2 recovery from the mouse brain. Limit of detection (LOD) and limit of quantification (LOQ) values of 0.5 and 2.5 pmol mL(-1), respectively, were reached for both 17 alpha-E-2 and 17 beta-E-2. LOD values for E-1 and E-3 were 0.01 and 0.025 pmol mL(-1), respectively. The variation coefficients for intra- and inter-assays were 6 and 14%, respectively, for both estradiol forms. The method was applied to assess estrogen levels in the mouse brain and hippocampus after 17 beta-E-2 acute (subcutaneous injection) and chronic (drinking water) physiological administration. Total estrogen levels were determined after enzymatic deconjugation and compared to free estrogen levels. While 17 alpha-E-2 was not detected in biological samples, 17 beta-E-2 and metabolite measurements highlight a local biotransformation of estrogens after physiological administration via drinking water.< Réduire