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Derivatization-free LC-MS/MS method for estrogen quantification in mouse brain highlights a local metabolic regulation after oral versus subcutaneous administration

hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLOZAN, Ecaterina
hal.structure.identifierNeurocentre Magendie-U862, Institut François Magendie, and Université de Bordeaux,
dc.contributor.authorSHINKARUK, Svitlana
hal.structure.identifierNeurocentre Magendie-U862, Institut François Magendie, and Université de Bordeaux,
dc.contributor.authorAL ABED, Alice Shaam
hal.structure.identifierNeurocentre Magendie-U862, Institut François Magendie, and Université de Bordeaux,
dc.contributor.authorLAMOTHE, Valerie
hal.structure.identifierNeurocentre Magendie-U862, Institut François Magendie, and Université de Bordeaux,
dc.contributor.authorPOTIER-GEORGES, Mylene
ORCID: 0000-0003-4077-6914
IDREF: 143044273
hal.structure.identifierNeurocentre Magendie-U862, Institut François Magendie, and Université de Bordeaux,
dc.contributor.authorMARIGHETTO, Aline
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorSCHMITTER, Jean-Marie
hal.structure.identifierNeurocentre Magendie-U862, Institut François Magendie, and Université de Bordeaux,
dc.contributor.authorBENNETAU, Catherine
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorBURE, Corinne
dc.date.accessioned2020-07-09T14:16:44Z
dc.date.available2020-07-09T14:16:44Z
dc.date.issued2017-09
dc.identifier.issn1618-2642
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/10292
dc.description.abstractEn17 beta-Estradiol (17 beta-E-2) is a steroid with pleiotropic actions. In addition to being a sexual hormone, it is also produced in the brain where it modulates the reproductive axis. It has been shown that 17 beta-E-2 also acts on synaptic plasticity and plays a role in neurological pathways and in neurodegenerative diseases. Assaying this steroid in the brain is thus interesting to improve our knowledge of 17 beta-E-2 effects in the brain. However, 17 beta-E-2 concentration in the central nervous system has been reported to be of a few nanograms per gram wet weight (nanomolar range concentration); therefore, its quantification requires both an efficient extraction process and a sensitive detection method. Herein is presented a derivatization-free procedure based on solid-phase extraction followed by LC-MS/MS analysis, targeted on 17 beta-E-2, its isomer17 alpha-E-2, and its metabolites estrone (E-1) and estriol (E-3). This extraction process allowed reaching 96% 17 beta-E-2 recovery from the mouse brain. Limit of detection (LOD) and limit of quantification (LOQ) values of 0.5 and 2.5 pmol mL(-1), respectively, were reached for both 17 alpha-E-2 and 17 beta-E-2. LOD values for E-1 and E-3 were 0.01 and 0.025 pmol mL(-1), respectively. The variation coefficients for intra- and inter-assays were 6 and 14%, respectively, for both estradiol forms. The method was applied to assess estrogen levels in the mouse brain and hippocampus after 17 beta-E-2 acute (subcutaneous injection) and chronic (drinking water) physiological administration. Total estrogen levels were determined after enzymatic deconjugation and compared to free estrogen levels. While 17 alpha-E-2 was not detected in biological samples, 17 beta-E-2 and metabolite measurements highlight a local biotransformation of estrogens after physiological administration via drinking water.
dc.title.enDerivatization-free LC-MS/MS method for estrogen quantification in mouse brain highlights a local metabolic regulation after oral versus subcutaneous administration
dc.typeArticle de revue
dc.identifier.doi10.1007/s00216-017-0473-9
dc.subject.halChimie/Matériaux
bordeaux.journalAnalytical and Bioanalytical Chemistry
bordeaux.page5279-5289
bordeaux.volume409
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248*
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248)
bordeaux.issue22
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionBordeaux INP
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Analytical%20and%20Bioanalytical%20Chemistry&rft.date=2017-09&rft.volume=409&rft.issue=22&rft.spage=5279-5289&rft.epage=5279-5289&rft.eissn=1618-2642&rft.issn=1618-2642&rft.au=LOZAN,%20Ecaterina&SHINKARUK,%20Svitlana&AL%20ABED,%20Alice%20Shaam&LAMOTHE,%20Valerie&POTIER-GEORGES,%20Mylene&rft.genre=article


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