COUSSOT, G.; FAYE, C.; IBRAHIM, A.; RAMONDA, M.; DOBRIJEVIC, M.; LE POSTOLLEC, A.; GRANIER, F.; VANDENABEELE-TRAMBOUZE, O.

doi:10.1007/s00216-010-4612-9

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COUSSOT, G.
Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM]

FAYE, C.
Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM]

IBRAHIM, A.
Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM]

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Analytical and Bioanalytical Chemistry. 2011, vol. 399, n° 6, p. 2295-2302

Springer Verlag

Résumé en anglais

The functionalization of surfaces with amino groups is used in many application areas such as in industrial biocatalytic processes for the development of medical biomaterials and in the environment for removing pollutants from water. Amino group density and grafting stability are often related to functionalized material performances; thus, their characterizations are of prime importance. The determination of amino density and grafting stability on polymeric material (e.g. polypropylene, polystyrene and cylco olefin copolymer) is often time consuming and sometimes presents technical constraints, more particularly with non-flat materials. In this paper, we report a novel colorimetric assay using the Coomassie Brilliant Blue dye for both amino density determination and grafting stability measurement. The assay named ADECA for "Amino Density Estimation by Colorimetric Assay" is sensitive, rapid, robust and versatile. We demonstrate that ADECA makes the evaluation of aminated materials performances possible for numerous material compositions, formats and chemistries used for grafting. Our study focuses on dendrigraft of poly-L-lysine and poly(amidoamine) dendrimers dendritic materials.< Réduire

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Colorimetric assay

Aminated surfaces

Solid support characterization

Dendrimers

Coating stability

ADECA

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Aminated dendritic surfaces characterization: a rapid and versatile colorimetric assay for estimating the amine density and coating stability

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