DEHOUX, Thomas; ABI GHANEM, Maroun; ZOUANI, Omar El-Farouk; RAMPNOUX, Jean-Michel; GUILLET, Yannick; DILHAIRE, Stefan; DURRIEU, Marie-Christine; AUDOIN, Bertrand

doi:10.1038/srep08650

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DEHOUX, Thomas

ABI GHANEM, Maroun

ZOUANI, Omar El-Farouk
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

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Article de revue

Ce document a été publié dans

Scientific Reports. 2015-03-03, vol. 5, p. 8650 (1-5)

Nature Publishing Group

Résumé en anglais

Cell mechanics play a key role in several fundamental biological processes, such as migration, proliferation, differentiation and tissue morphogenesis. In addition, many diseased conditions of the cell are correlated with altered cell mechanics, as in the case of cancer progression. For this there is much interest in methods that can map mechanical properties with a sub-cell resolution. Here, we demonstrate an inverted pulsed opto-acoustic microscope (iPOM) that operates in the 10 to 100 GHz range. These frequencies allow mapping quantitatively cell structures as thin as 10 nm and resolving the fibrillar details of cells. Using this non-invasive all-optical system, we produce high-resolution images based on mechanical properties as the contrast mechanisms, and we can observe the stiffness and adhesion of single migrating stem cells. The technique should allow transferring the diagnostic and imaging abilities of ultrasonic imaging to the single-cell scale, thus opening new avenues for cell biology and biomaterial sciences.< Réduire

Mots clés en anglais

Biosensors

Cellular Imaging

URI

https://oskar-bordeaux.fr/handle/20.500.12278/77875

DOI

http://dx.doi.org/10.1038/srep08650

Project ANR

Imagerie quantitative par acoustique picoseconde de l'adhésion de cellules individuelles sur biomatériaux - ANR-13-BS09-0021

Origine

Importé de hal

Unités de recherche

Institut de Mécanique et d’Ingénierie de Bordeaux (I2M) - UMR 5295