LEGRAND, Anthony; MARTINEZ, Denis; GRELARD, Axelle; BERBON, Melanie; MORVAN, Estelle; TAWANI, Arpita; LOQUET, Antoine; MONGRAND, Sébastien; HABENSTEIN, Birgit

doi:10.3389/fmolb.2019.00107

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LEGRAND, Anthony

Laboratoire de biogenèse membranaire [LBM]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

MARTINEZ, Denis

Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

GRELARD, Axelle
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

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Article de revue

Ce document a été publié dans

Frontiers in Molecular Biosciences. 2019, vol. 6

Résumé en anglais

Nanodomains are dynamic membrane subcompartments, enriched in specific lipid, and protein components that act as functional platforms to manage an abundance of cellular processes. The remorin protein of plants is a well-established nanodomain marker and widely serves as a paradigm to study nanodomain clustering. Located at the inner leaflet of the plasma membrane, remorins perform essential functions during signaling. Using deuteriumand phosphorus solid-state NMR, we inquire on themolecular determinants of the lipid-protein and protein-protein interactions driving nanodomain clustering. By monitoring thermotropism properties, lipid acyl chain order and membrane thickness, we report the effects of phosphoinositides and sterols on the interaction of various remorin peptides and protein constructs with the membrane. We probed several critical residues involved in this interaction and the involvement of the coiled-coil homo-oligomerisation domain into the formation of remorin nanodomains. We trace the essential role of the pH in nanodomain clustering based on anionic lipids such as phosphoinositides. Our results reveal a complex interplay between specific remorin residues and domains, the environmental pH and their resulting effects on the lipid dynamics for phosphoinositide-enriched membranes.< Réduire

Project ANR

Facteurs cellulaires recrutés par les potyvirus pour leur transport intercellulaire : de nouvelles sources de résistance des plantes? - ANR-16-CE20-0008

Unités de recherche

CBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248