BOURGEOIS, Christine; GOMAA, Ahmed I.; LEFEVRE, Thierry; CANSELL, Maud; SUBIRADE, Muriel

doi:10.1016/j.ijbiomac.2018.10.211

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

BOURGEOIS, Christine
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

GOMAA, Ahmed I.

LEFEVRE, Thierry

Langue

Article de revue

Ce document a été publié dans

International journal of biological macromolecules. 2018, vol. 122, p. 873-881

Résumé en anglais

It is crucial to develop new natural sources of emulsifiers to substitute the synthetic molecules. An ideal emulsifying system exists in plants that is consisting of oil bodies proteins and phospholipids. In this study, Fourier transformed infrared (FTIR) spectroscopy was used to investigate the interactions between oil bodies proteins (OBP) and model phospholipid (PL) membranes. The secondary structure and PL thermotropism were investigated. Different PL varying in chain length and polar head were used including two zwitterionic phospholipids, dimyristoylphosphatidylcholine and dioleoylphosphatidylcholine, and two anionic phospholipids, dimyristoylphosphatidylglycerol, dipalmitoylphosphatidylglycerol. The changes in lipid physical state and protein denaturation were investigated as a function of temperature from 20 to 80 °C. OBP in solution is composed of unordered structures and beta-sheets with signs of aggregation. Anionic PL interacts with OBP whereas zwitterionic PL does not or only slightly interacts with the protein. Unsaturated PL promoted the alpha-helix structure in OBP. The interactions between OBP and PL depended on the protein charge inducing different protein conformations. Overall, the study showed that OBP and commercial anionic phospholipids have a potential in developing stable emulsifier for food industry.< Réduire

Mots clés en anglais

Oil body proteins

Phospholipids

Emulsions

FTIR

Métadonnées

Partager cette publication !

Licence d’utilisation du document

Interaction of oil bodies proteins with phospholipid bilayers: A molecular level elucidation as revealed by infrared spectroscopy

Langue

Ce document a été publié dans

Résumé en anglais

Mots clés en anglais

URI

DOI

Unités de recherche