DALLET, Laurence; DECOSSAS, Marion; TAVEAU, Jean-Christophe; LECOMTE, Sophie; POUSSARD, Sylvie; LAMBERT, Olivier; PITARD, Bruno

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DALLET, Laurence

Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

DECOSSAS, Marion
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

TAVEAU, Jean-Christophe

Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

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Article de revue

Ce document a été publié dans

Nanomedicine-Nanotechnology Biology and Medicine. 2018, vol. 14, n° 1, p. 141-151

Résumé en anglais

Delivery of biologically active proteins into cells is emerging as important strategy for many applications. Previous experiments have shown that lipoaminoglycosides were capable of delivery of the anti-cytokeratin8 antibody (anti-K8) but only when formulated with lipid helpers potentially leading to toxicity from excess lipids. Here, we optimized anti-K8 delivery with various lipoaminoglycosides in the absence of a lipid helper. Results led to the identification of the aminoglycoside lipid dioleyl phosphoramido ribostamycin (DOPRI) as a potent intracellular delivery system for anti-K8. Electron microscopy revealed that delivered anti-K8 molecules were bound to intermediate filaments in cells. Anti-K8 was bound to the surface of DOPRI vesicles without perturbing lipid organization. Macropinocytosis and caveolin mediated endocytosis contributed to anti-K8 internalization and to filament labeling with a major contribution being made by the caveolin pathway. The results showed that the unique properties of DOPRI were sufficient for efficient intracellular protein delivery without requiring lipid helpers. (C) 2017 Elsevier Inc. All rights reserved.< Réduire

Mots clés en anglais

Antibodies

Lipoaminoglycosides

Intracellular delivery

Supramolecular structure

URI

https://oskar-bordeaux.fr/handle/20.500.12278/4281

Unités de recherche

CBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248