Caveolae-mediated effects of TNF-alpha on human skeletal muscle cells
Langue
EN
Article de revue
Ce document a été publié dans
Experimental Cell Research. 2018, vol. 370, n° 2, p. 623-631
Résumé en anglais
Chronic diseases are characterized by the production of pro-inflammatory cytokines such than TNF-alpha and are frequently correlated with muscle wasting conditions. Among the pleiotropic effects of TNF-alpha within the ...Lire la suite >
Chronic diseases are characterized by the production of pro-inflammatory cytokines such than TNF-alpha and are frequently correlated with muscle wasting conditions. Among the pleiotropic effects of TNF-alpha within the cell, its binding to TNFR1 receptor has been shown to activate sphingomyelinases leading to the production of ceramides. Sphingomyelinases and TNF receptor have been localized within caveolae which are specialized RAFT enriched in cholesterol and sphingolipids. Because of their inverted omega shape, maintained by the oligomerization of specialized proteins, caveolins and cavins, caveolae serve as membrane reservoir therefore providing mechanical protection to plasma membranes. Although sphingolipids metabolites, caveolins and TNF-alpha/TNFR1 have been shown to independently interfere with muscle physiology, no data have clearly demonstrated their concerted action on muscle cell regeneration. In this context, our study aimed at studying the molecular mechanisms induced by TNF-alpha at the level of caveolae in LHCN-M2 human muscle satellite cells. Here we showed that TNF-alpha-induced production of ROS and nSMase activation requires caveolin. More strikingly, we have demonstrated that TNF-alpha induces the formation of additional caveolae at the plasma membrane of myoblasts. Furthermore, TNF-alpha prevents myoblast fusion suggesting that inflammation could modulate caveolae organization/function and satellite cell function.< Réduire
Mots clés en anglais
Caveolae
Myoblasts
Inflammation
TNF-α
Oxidative stress
Sphingomyelinase
Unités de recherche