MARTINEZ, Denis; LEGRAND, Anthony; GRONNIER, Julien; DECOSSAS, Marion; GOUGUET, Paul; LAMBERT, Olivier; BERBON, Melanie; VERRON, Loris; GRELARD, Axelle; GERMAIN, Veronique; LOQUET, Antoine; MONGRAND, Sébastien; HABENSTEIN, Birgit

doi:10.1016/j.jsb.2018.02.003

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

MARTINEZ, Denis

Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

LEGRAND, Anthony

Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

GRONNIER, Julien
Laboratoire de biogenèse membranaire [LBM]

Langue

Article de revue

Ce document a été publié dans

Journal of Structural Biology. 2018, vol. 206, n° 1, p. 12-19

Résumé en anglais

REMORINs are nanodomain-organized proteins located in the plasma membrane and involved in cellular responses in plants. The dynamic assembly of the membrane nanodomains represents an essential tool of the versatile membrane barriers to control and modulate cellular functions. Nevertheless, the assembly mechanisms and protein organization strategies of nanodomains are poorly understood and many structural aspects are difficult to visualize. Using an ensemble of biophysical approaches, including solid-state nuclear magnetic resonance, cryo-electron microscopy and in vivo confocal imaging, we provide first insights on the role and the structural mechanisms of REMORIN trimerization. Our results suggest that the formation of REMORIN coiled-coil trimers is essential for membrane recruitment and promotes REMORIN assembly in vitro into long filaments by trimer-trimer interactions that might participate in nanoclustering into membrane domains in vivo.< Réduire

Mots clés en anglais

REMORIN

Nanodomain

Membrane protein

Solid-state NMR

Protein filament

Métadonnées

Partager cette publication !

Licence d’utilisation du document

Coiled-coil oligomerization controls localization of the plasma membrane REMORINs

Langue

Ce document a été publié dans

Résumé en anglais

Mots clés en anglais

URI

DOI

Unités de recherche