Comparative study of strong cation exchangers: Structure-related chromatographic performances
Langue
EN
Article de revue
Ce document a été publié dans
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 2018, vol. 1080, p. 1-10
Résumé en anglais
Chromatographic performances are highly influenced by operational parameters. New ion exchangers have tailored matrices providing low backpressure, thereby allowing high flow velocity. By systematic frontal analysis and ...Lire la suite >
Chromatographic performances are highly influenced by operational parameters. New ion exchangers have tailored matrices providing low backpressure, thereby allowing high flow velocity. By systematic frontal analysis and selectivity determination at different flow rates, we independently evaluated cation exchangers to facilitate media selection and investigated the relationship between surface modification and chromatographic performances. Structure-extended resins showed higher binding capacities compared to resins with conventional ligands directly attached to the matrix. Moreover, they maintained high capacities even with high flow velocities. Ligand accessibility was therefore largely enhanced, allowing proteins to interact and bind under harsh conditions with minimal residence/contact time. High throughput resins can be used for purification of high volume and high concentration feedstock in limited time. This results in higher productivity, and could contribute to cost reduction. In this work, we evaluated the dynamic binding capacities of various new ion exchange resins at different binding conductivities for different residence times, and observed that.< Réduire
Mots clés en anglais
Cation exchange
Resolution
Dynamic binding capacity
Flow rate
Unités de recherche