PERRAKI, A.; GRONNIER, J.; GOUGUET, P.; BOUDSOCQ, M.; DEROUBAIX, A. F.; SIMON, V.; GERMAN-RETANA, S.; LEGRAND, A.; HABENSTEIN, Birgit; ZIPFEL, C.; BAYER, Emmanuelle; MONGRAND, Sébastien; GERMAIN, Veronique

doi:10.1371/journal.ppat.1007378

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PERRAKI, A.
Laboratoire de biogenèse membranaire [LBM]

GRONNIER, J.
Laboratoire de biogenèse membranaire [LBM]

GOUGUET, P.
Laboratoire de biogenèse membranaire [LBM]

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Article de revue

Ce document a été publié dans

Plos Pathogens. 2018, vol. 14, n° 11

Résumé en anglais

Plants respond to pathogens through dynamic regulation of plasma membrane-bound signaling pathways. To date, how the plant plasma membrane is involved in responses to viruses is mostly unknown. Here, we show that plant cells sense the Potato virus X (PVX) COAT PROTEIN and TRIPLE GENE BLOCK 1 proteins and subsequently trigger the activation of a membrane-bound calcium-dependent kinase. We show that the Arabidopsis thaliana CALCIUM-DEPENDENT PROTEIN KINASE 3-interacts with group 1 REMORINs in vivo, phosphorylates the intrinsically disordered N-terminal domain of the Group 1 REMORIN REM1.3, and restricts PVX cell-to-cell movement. REM1.3's phospho-status defines its plasma membrane nanodomain organization and is crucial for REM1.3-dependent restriction of PVX cell-to-cell movement by regulation of callose deposition at plasmodesmata. This study unveils plasma membrane nanodomain-associated molecular events underlying the plant immune response to viruses.< Réduire

Mots clés en anglais

Phosphorylation

Leaves

In vitro kinase assay

Arabidopsis thaliana

Protein extraction

Membrane proteins; Cell membranes

Agrobacterium tumefaciens

Projet Européen

Signaling initiation and specificity in BAK1-dependent receptor kinase-mediated innate immunity in Arabidopsis

Project ANR

Facteurs cellulaires recrutés par les potyvirus pour leur transport intercellulaire : de nouvelles sources de résistance des plantes?

Unités de recherche

CBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248