BERTHELOT, Karine; ESTEVEZ, Yannick; QUILIANO, Miguel; BALDERA-AGUAYO, Pedro A; ZIMIC, Mirko; PRIBAT, Anne; BAKLEH, Marc-Elias; TEYSSIER, Emeline; GALLUSCI, Philippe; GARDRAT, Christian; LECOMTE, Sophie; PERUCH, Frédéric

doi:10.1016/j.biochi.2016.05.005

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

BERTHELOT, Karine
Laboratoire de Chimie des Polymères Organiques [LCPO]

ESTEVEZ, Yannick
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

QUILIANO, Miguel

Langue

Article de revue

Ce document a été publié dans

Biochimie. 2016, vol. 127, p. 133-43

Résumé en anglais

In this study, we cloned, expressed and purified the isopentenyl diphosphate isomerases (IDIs) from two plants, Hevea brasiliensis and Solanum lycopersicum, and compared them to the already well characterized Escherichia coli IDI. Phylogenetic analysis showed high homology between the three enzymes. Their catalytic activity was investigated in vitro with recombinant purified enzymes and in vivo by complementation colorimetric tests. The three enzymes displayed consistent activities both in vitro and in vivo. In term of structure, studied by ATR-FTIR and molecular modeling, it is clear that both plant enzymes are more related to their human homologue than to E. coli IDI. But it is assumed that EcIDI represent the minimalistic part of the catalytic core, as both plant enzymes present a supplementary sequence forming an extra α-helice surrounding the catalytic site that could facilitate the biocatalysis. New potential biotechnological applications may be envisaged.< Réduire

Mots clés en anglais

Atr-Ftir

Enzyme Structure-Activity

Hevea Brasiliensis

Isopentenyl Diphosphate Isomerase

Isoprenoid Biosynthesis

Solanum Lycopersicum

URI

https://oskar-bordeaux.fr/handle/20.500.12278/4000

DOI

http://dx.doi.org/10.1016/j.biochi.2016.05.005

Métadonnées

Licence d’utilisation du document

HbIDI, SlIDI and EcIDI: A comparative study of isopentenyl diphosphate isomerase activity and structure.

Langue

Ce document a été publié dans

Résumé en anglais

Mots clés en anglais

URI

DOI

Unités de recherche

Métadonnées

Partager cette publication !

Licence d’utilisation du document

HbIDI, SlIDI and EcIDI: A comparative study of isopentenyl diphosphate isomerase activity and structure.

Langue

Ce document a été publié dans

Résumé en anglais

Mots clés en anglais

URI

DOI

Unités de recherche