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Checkup, a Rapid Tool for Detecting Chromosomal Rearrangements Related to the Promoter in : An Ecological and Technological Study on Wine Yeast.

dc.rights.licenseopenen_US
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorMARULLO, Philippe
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorCLAISSE, Olivier
ORCID: 0000-0002-6273-6289
IDREF: 134190726
dc.contributor.authorRAYMOND EDER, Maria Laura
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorBÖRLIN, Marine
dc.contributor.authorFEGHALI, Nadine
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorBERNARD, Margaux
dc.contributor.authorLEGRAS, Jean-Luc
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorALBERTIN, Warren
dc.contributor.authorROSA, Alberto Luis
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorMASNEUF POMAREDE, Isabelle
ORCID: 0000-0002-8806-8944
IDREF: 13239667X
dc.date.accessioned2020-12-03T17:00:56Z
dc.date.available2020-12-03T17:00:56Z
dc.date.issued2020-01-01
dc.identifier.issn1664-302Xen_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/21289
dc.description.abstractEnChromosomal rearrangements (CR) such as translocations, duplications and inversions play a decisive role in the adaptation of microorganisms to specific environments. In enological strains, CR involving the promoter region of the gene lead to a higher sulfite tolerance by enhancing the SO efflux. To date, three different associated CR events have been described, including translocations XV-t-XVI and VIII-t-XVI and inversion inv-XVI. In the present study, we developed a multiplex PCR method ( checkup) that allows a rapid characterization of these three chromosomal configurations in a single experiment. Nearly 600 strains collected from fermented grape juice were genotyped by microsatellite markers. We demonstrated that alleles of the promoter are differently distributed according to the wine environment (cellar versus vineyard) and the nature of the grape juice. Moreover, rearranged promoters are significantly enriched among commercial starters. In addition, the analysis of nearly isogenic strains collected in wine related environments demonstrated that the inheritance of these CR shapes the genetic diversity of clonal populations. Finally, the link between the nature of promoter and the tolerance to sulfite was statistically validated in natural grape juice containing various SO concentrations. The checkup is therefore a convenient new tool for addressing population genetics questions and for selecting yeast strains by using molecular markers.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.title.enCheckup, a Rapid Tool for Detecting Chromosomal Rearrangements Related to the Promoter in : An Ecological and Technological Study on Wine Yeast.
dc.title.alternativeFront Microbiolen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.3389/fmicb.2020.01331en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
dc.identifier.pubmed32695077en_US
bordeaux.journalFrontiers in Microbiologyen_US
bordeaux.page1331en_US
bordeaux.volume11en_US
bordeaux.hal.laboratoriesOenologie - EA 4577en_US
bordeaux.institutionBordeaux Sciences Agroen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.identifier.funderIDFondo para la Investigación Científica y Tecnológicaen_US
bordeaux.identifier.funderIDUniversidad Católica de Córdobaen_US
bordeaux.import.sourcepubmed
hal.exportfalse
workflow.import.sourcepubmed
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Frontiers%20in%20Microbiology&rft.date=2020-01-01&rft.volume=11&rft.spage=1331&rft.epage=1331&rft.eissn=1664-302X&rft.issn=1664-302X&rft.au=MARULLO,%20Philippe&CLAISSE,%20Olivier&RAYMOND%20EDER,%20Maria%20Laura&B%C3%96RLIN,%20Marine&FEGHALI,%20Nadine&rft.genre=article


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