'Candidatus Phytoplasma solani' (CPs) is an etiological agent of the grapevine ‘bois noir’ (BN) disease as well as similar diseases affecting many other cultivated plants and causing significant economic losses. CPs is endemic in the Euro-Mediterranean zone, but can be sporadically detected worldwide. It circulates in different pathosystem consisting of cultivated plants, polyphagous cixiid planthopper vectors and reservoir plants, often weeds, in nature. Involvement of different CPs strains in BN disease and their different epidemiological cycles can be revealed through multilocus sequence typing (MLST). Genes tuf, encoding the translation elongation factor Tu, secY, coding for a translocation protein, and two for variable surface proteins vmp1 and stamp are frequently used to asses BN genetic diversity and thus better inform the BN disease control strategies. In this study, CPs genetic diversity research in over a decade is presented. Two new stamp (ST59) and vmp1 (V28) CPs genotypes were revealed in the grapevine and totally 28 different MLST genotypes with the prevalence of CPsSqt21 (S6/ST6/V-18/tuf-b2) type linked to Hyalesthes obsoletus vector and Urtica dioica reservoir plant. Other two important genotypes for the grapevine are CPsSqt28 (S39/ST46/V3/tuf-a) and CPsSqtT2 (S1/ST9/V4/tuf-b1) associated respectively with U. dioica and Convolvulus arvensis. Cixius wagneri vector harbors yet different vmp1 CPs genotypes all over the county. CPs was also detected in Dicthyophara europaea insect and two new potential reservoirs (Ailanthus altissima, Robinia pseudoacacia). With such high level of CPs genetic variability, it is evident that several independent cycles have to be considered regarding the BN epidemiology in Croatia, a country with unique geographical characteristics covering different eco-climatic zones within south-east Europe.Read less <