not well known. We used a multi-satellite approach to quantify the surface water storage variations over the 2000-2020 period and relate these variations to climate-induced and anthropogenic factors over the whole basin. Our results highlight that dam operations have strongly modified the water regime of the Mekong River, exhibiting a 55 % decrease in the seasonal cycle amplitude of inundation extent (from 3178 km 2 to 1414 km 2 ) and a 70 % decrease in surface water volume (from 1109 km 3 to 327 km 3 ) over 2000-2020. In the floodplains of the Lower Mekong Basin, where rice is cultivated, there has been a decline in water residence time by 30 to 50 days. The recent commissioning of big dams (2010 and 2014) has allowed us to choose 2015 as a turning point year. Results show a trend inversion in rice production, from a rise of 40 % between 2000 and 2014 to a decline of 10 % between 2015 and 2020, and a strong reduction in aquaculture growth, from +730 % between 2000 and 2014, to +53 % between 2015 and 2020. All these results show the negative impact of dams on the Mekong basin, causing a 70 % decline in surface water volumes, with major repercussions for agriculture and fisheries over the period 2000-2020. Therefore, new future projects such as the Funan Techo canal in Cambodia, scheduled to start construction at the end of 2024, will particularly affect 1300 km 2 of floodplains in the lower Mekong basin, with a reduction in the amount of water received, and other areas will be subjected to flooding. The human, material and economic damage could be catastrophic.Read less <