GOUNOU, Celine; ROUYER, Lucile; SIEGFRIED, Geraldine; HARTE, Etienne; BOUVET, Flora; D'AGATA, Lena; DARBO, Elodie; LEFEUVRE, Martin; DERIEPPE, M; BOUTON, Lea; MELANE, Mailys; CHAPEAU, Dorian; MARTINEAU, Julie; PROUZET-MAULEON, Valerie; TAN, Sisareuth; SOULEYREAU, Wilfried; SALTEL, Frederic; ARGOUL, Francoise; KHATIB, Abdel‐majid; BRISSON, Alain; IGGO, Richard; BOUTER, Anthony

doi:10.1007/s00018-023-05049-3

GOUNOU, Celine
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

ROUYER, Lucile

SIEGFRIED, Geraldine

Université de Bordeaux [UB]

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Article de revue

Ce document a été publié dans

Cellular and Molecular Life Sciences. 2023-12-13, vol. 81, n° 1, p. 7

Résumé en anglais

Cancer cells are exposed to major compressive and shearing forces during invasion and metastasis, leading to extensive plasma membrane damage. To survive this mechanical stress, they need to repair membrane injury efficiently. Targeting the membrane repair machinery is thus potentially a new way to prevent invasion and metastasis. We show here that annexin-A2 (ANXA2) is required for membrane repair in invasive breast and pancreatic cancer cells. Mechanistically, we show by fluorescence and electron microscopy that cells fail to reseal shear-stress damaged membrane when ANXA2 is silenced or the protein is inhibited with neutralizing antibody. Silencing of ANXA2 has no effect on proliferation in vitro, and may even accelerate migration in wound healing assays, but reduces tumor cell dissemination in both mice and zebrafish. We expect that inhibiting membrane repair will be particularly effective in aggressive, poor prognosis tumors because they rely on the membrane repair machinery to survive membrane damage during tumor invasion and metastasis. This could be achieved either with anti-ANXA2 antibodies, which have been shown to inhibit metastasis of breast and pancreatic cancer cells, or with small molecule drugs.< Réduire

Mots clés en anglais

Annexins

Invasion

Metastasis

Tumor progression

S100 proteins

MDA-MB-231

AsPC-1

URI

https://oskar-bordeaux.fr/handle/20.500.12278/204003

DOI

http://dx.doi.org/10.1007/s00018-023-05049-3

Unités de recherche

CBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248

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Inhibition of the membrane repair protein annexin-A2 prevents tumor invasion and metastasis

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Résumé en anglais

Mots clés en anglais

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