Abstract The recent biological invasion of box tree moth Cydalima perspectalis on Buxus trees has a major impact on European boxwood stands through severe defoliation. This can hinder further regrowth and threaten survival of populations. In a mesocosm approach and controlled larval density over a 2‐month period, responses of B. sempervirens essential and specialized metabolites were characterized using metabolomics, combining 1 H–NMR and LC–MS/MS approaches. This is the first metabolome depiction of major Buxus responses to boxwood moth invasion. Under severe predation, remaining green leaves accumulate free amino acids (with the noticeable exception of proline). The leaf trans‐4‐hydroxystachydrine and stachydrine reached 10–13% and 2–3% (DW), while root content was lower but also modulated by predation level. Larval predation promoted triterpenoid and (steroidal) alkaloid synthesis and diversification, while flavonoids did not seem to have a relevant role in Buxus resistance. Our results reveal the concomitant responses of central and specialized metabolism, in relation to severity of predation. They also confirm the potential of metabolic profiling using 1 H–NMR and LC–MS to detect re‐orchestration of metabolism of native boxwood after severe herbivorous predation by the invasive box‐tree moth, and thus their relevance for plant–insect relationships and ecometabolomics.Read less <