GUIRAUD, Jennifer; HELARY, Marion; LE ROY, Chloé; ELGUERO, Eric; PEREYRE, Sabine; BÉBÉAR, Cécile

doi:10.3390/microorganisms10081587

GUIRAUD, Jennifer
Microbiologie Fondamentale et Pathogénicité [MFP]

HELARY, Marion
Microbiologie Fondamentale et Pathogénicité [MFP]

LE ROY, Chloé
Microbiologie Fondamentale et Pathogénicité [MFP]

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Article de revue

Ce document a été publié dans

Microorganisms. 2022-08-06, vol. 10, n° 8

Résumé en anglais

Mycoplasma genitalium causes sexually transmitted infecti.ons in men and women. Treatment failures to macrolides and fluoroquinolones have been reported worldwide. Although the mgpB typing method has often been used in M. genitalium-infected men who have sex with men (MSM), limited typing data are available for M. genitalium-infected women. In this study, we aimed to investigate the genetic relationship between M. genitalium strains and their antibiotic resistance profile in a cohort of MSM (86.2% on HIV preexposure prophylaxis [PrEP], 13.8% HIV positive) and a large cohort of women using mgpB/MG309 typing. The mgpB types were determined in 374 samples from 305 women and 65 MSM. Three MSM and one woman had two concurrent or subsequent samples. Macrolide and fluoroquinolone resistance-associated mutations were searched in the 23S rRNA as well as parC and gyrA genes. The mgpB phylogenetic construction revealed three large clusters that differed according to sexual practices and geographical origin of patients. The prevalence of macrolide and fluoroquinolone resistance was significantly higher in MSM compared with women (95.4% vs. 14.1% and 30.6% vs. 7.2%, p < 0.001, respectively). The macrolide resistance spread was polyclonal in both populations, but clonal diffusion of two dual-resistant types was observed in PrEP users in association with high antibiotic pressure and dense connectivity in this population.< Réduire

Mots clés en anglais

MG309; Mycoplasma genitalium; antimicrobial resistance; fluoroquinolones; macrolides; mgpB; sexually transmitted infection; typing.

URI

https://oskar-bordeaux.fr/handle/20.500.12278/203681

DOI

http://dx.doi.org/10.3390/microorganisms10081587

Unités de recherche

MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234

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Molecular Typing Reveals Distinct Transmission Networks among a Cohort of Men Who Have Sex with Men and a Cohort of Women in France.

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