A stable microtubule array drives fission yeast polarity reestablishment upon quiescence exit
BROCARD, Lysiane
Biologie du fruit et pathologie [BFP]
Institut National de la Recherche Agronomique [INRA]
Université de Bordeaux [UB]
< Réduire
Biologie du fruit et pathologie [BFP]
Institut National de la Recherche Agronomique [INRA]
Université de Bordeaux [UB]
Langue
en
Article de revue
Ce document a été publié dans
Journal of Cell Biology. 2015-07-06, vol. 210, n° 1, p. 99-113
Rockefeller University Press
Résumé en anglais
Cells perpetually face the decision to proliferate or to stay quiescent. Here we show that upon quiescence establishment, Schizosaccharomyces pombe cells drastically rearrange both their actin and microtubule (MT) cytoskeletons ...Lire la suite >
Cells perpetually face the decision to proliferate or to stay quiescent. Here we show that upon quiescence establishment, Schizosaccharomyces pombe cells drastically rearrange both their actin and microtubule (MT) cytoskeletons and lose their polarity. Indeed, while polarity markers are lost from cell extremities, actin patches and cables are reorganized into actin bodies, which are stable actin filament–containing structures. Astonishingly, MTs are also stabilized and rearranged into a novel antiparallel bundle associated with the spindle pole body, named Q-MT bundle. We have identified proteins involved in this process and propose a molecular model for Q-MT bundle formation. Finally and importantly, we reveal that Q-MT bundle elongation is involved in polarity reestablishment upon quiescence exit and thereby the efficient return to the proliferative state. Our work demonstrates that quiescent S. pombe cells assemble specific cytoskeleton structures that improve the swiftness of the transition back to proliferation.< Réduire
Mots clés en anglais
benzimidazol-2-yl-carbamate
MPA
mycophenolic acid
MT
microtubule
MTOC
MT organizing center
SPB
benzimidazol-2-yl-carbamate MPA
mycophenolic acid MT
microtubule MTOC
MT organizing center SPB
spindle pole
Origine
Importé de halUnités de recherche