AL SAYED, Zeina R; JOUVE, Charlene; SEGURET, Magali; RUIZ-VELASCO, Andrea; PEREIRA, Celine; TREGOUET, David-Alexandre; HULOT, Jean-Sebastien

doi:10.1016/j.stemcr.2024.08.005

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Stem Cell Reports. 2024-10-08, vol. 19, n° 10, p. 1417-1431

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Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) offer great potential for drug screening and disease modeling. However, hiPSC-CMs remain immature compared to the adult cardiac cells. Cardiomyocytes isolated from adult human hearts have a typical rod-shaped morphology. Here, we sought to develop a simple method to improve the architectural maturity of hiPSC-CMs by using a rod-shaped cell micropatterned substrate consisting of repeated rectangles (120 μm long × 30 μm wide) surrounded by a chemical cell repellent. The generated hiPSC-CMs exhibit numerous characteristics similar to adult human cardiomyocytes, including elongated cell shape, well-organized sarcomeres, and increased myofibril density. The improvement in structural properties correlates with the enrichment of late ventricular action potentials characterized by a more hyperpolarized resting membrane potential and an enhanced depolarization consistent with an increased sodium current density. The more mature hiPSC-CMs generated by this method may serve as a useful in vitro platform for characterizing cardiovascular disease.< Leer menos

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https://oskar-bordeaux.fr/handle/20.500.12278/202556

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http://dx.doi.org/10.1016/j.stemcr.2024.08.005

Proyecto ANR

Édition génomique par CRISPR/Cas9 pour traiter les cardiomyopathies liées aux mutations du gène LMNA - ANR-19-CE17-0013

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Bordeaux Population Health Research Center (BPH) - UMR 1219

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Rod-shaped micropatterning enhances the electrophysiological maturation of cardiomyocytes derived from human induced pluripotent stem cells

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