Rod-shaped micropatterning enhances the electrophysiological maturation of cardiomyocytes derived from human induced pluripotent stem cells
| dc.rights.license | open | en_US |
| dc.contributor.author | AL SAYED, Zeina R | |
| dc.contributor.author | JOUVE, Charlene | |
| dc.contributor.author | SEGURET, Magali | |
| dc.contributor.author | RUIZ-VELASCO, Andrea | |
| dc.contributor.author | PEREIRA, Celine | |
| hal.structure.identifier | Bordeaux population health [BPH] | |
| dc.contributor.author | TREGOUET, David-Alexandre | |
| dc.contributor.author | HULOT, Jean-Sebastien | |
| dc.date.accessioned | 2024-10-17T09:29:36Z | |
| dc.date.available | 2024-10-17T09:29:36Z | |
| dc.date.issued | 2024-10-08 | |
| dc.identifier.issn | 2213-6711 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/202556 | |
| dc.description.abstractEn | Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) offer great potential for drug screening and disease modeling. However, hiPSC-CMs remain immature compared to the adult cardiac cells. Cardiomyocytes isolated from adult human hearts have a typical rod-shaped morphology. Here, we sought to develop a simple method to improve the architectural maturity of hiPSC-CMs by using a rod-shaped cell micropatterned substrate consisting of repeated rectangles (120 μm long × 30 μm wide) surrounded by a chemical cell repellent. The generated hiPSC-CMs exhibit numerous characteristics similar to adult human cardiomyocytes, including elongated cell shape, well-organized sarcomeres, and increased myofibril density. The improvement in structural properties correlates with the enrichment of late ventricular action potentials characterized by a more hyperpolarized resting membrane potential and an enhanced depolarization consistent with an increased sodium current density. The more mature hiPSC-CMs generated by this method may serve as a useful in vitro platform for characterizing cardiovascular disease. | |
| dc.description.sponsorship | Édition génomique par CRISPR/Cas9 pour traiter les cardiomyopathies liées aux mutations du gène LMNA - ANR-19-CE17-0013 | en_US |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
| dc.title.en | Rod-shaped micropatterning enhances the electrophysiological maturation of cardiomyocytes derived from human induced pluripotent stem cells | |
| dc.title.alternative | Stem Cell Reports | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1016/j.stemcr.2024.08.005 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Santé publique et épidémiologie | en_US |
| dc.identifier.pubmed | 39303707 | en_US |
| bordeaux.journal | Stem Cell Reports | en_US |
| bordeaux.page | 1417-1431 | en_US |
| bordeaux.volume | 19 | en_US |
| bordeaux.hal.laboratories | Bordeaux Population Health Research Center (BPH) - UMR 1219 | en_US |
| bordeaux.issue | 10 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | INSERM | en_US |
| bordeaux.team | ELEANOR_BPH | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.identifier.funderID | Fondation Leducq | en_US |
| bordeaux.identifier.funderID | Fondation pour la Recherche Médicale | en_US |
| hal.identifier | hal-04741122 | |
| hal.version | 1 | |
| hal.date.transferred | 2024-10-17T09:29:40Z | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | true | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Stem%20Cell%20Reports&rft.date=2024-10-08&rft.volume=19&rft.issue=10&rft.spage=1417-1431&rft.epage=1417-1431&rft.eissn=2213-6711&rft.issn=2213-6711&rft.au=AL%20SAYED,%20Zeina%20R&JOUVE,%20Charlene&SEGURET,%20Magali&RUIZ-VELASCO,%20Andrea&PEREIRA,%20Celine&rft.genre=article |
