Membrane properties of giant polymer and lipid vesicles obtained by electroformation and PVA gel-assisted hydration methods
DAO, Thi Phuong Tuyen
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
FERNANDES, Fabio
Univ Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol
Univ Lisbon, Inst Super Tecn, Ctr Quim Fis Mol
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Univ Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol
Univ Lisbon, Inst Super Tecn, Ctr Quim Fis Mol
DAO, Thi Phuong Tuyen
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
FERNANDES, Fabio
Univ Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol
Univ Lisbon, Inst Super Tecn, Ctr Quim Fis Mol
Univ Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol
Univ Lisbon, Inst Super Tecn, Ctr Quim Fis Mol
IBARBOURE, Emmanuel
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
PRIETO, Manuel
Univ Lisbon, Inst Super Tecn, Ctr Quim Fis Mol
Univ Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol
Univ Lisbon, Inst Super Tecn, Ctr Quim Fis Mol
Univ Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol
LE MEINS, Jean-Francois
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Laboratoire de Chimie des Polymères Organiques [LCPO]
< Reduce
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Laboratoire de Chimie des Polymères Organiques [LCPO]
Language
en
Article de revue
This item was published in
Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2017, vol. 553, p. 347-353
Elsevier
English Abstract
Giant Unilamellar Vesicles (GUV) are the most commonly used biomimetic membrane model. Their production in physiological conditions through the well-known electroformation process is complex and other gel-assisted hydration ...Read more >
Giant Unilamellar Vesicles (GUV) are the most commonly used biomimetic membrane model. Their production in physiological conditions through the well-known electroformation process is complex and other gel-assisted hydration procedures have recently emerged to circumvent some limitations of the process. In this work, the membrane physical properties of Giant Polymer Unilamellar Vesicles (Polymersomes) and lipid vesicles (Liposomes) obtained from the gel-assisted hydration procedure and electroformation, have been evaluated thoroughly by different techniques: micropipettes aspiration (MPA), Fluorescence Recovery After Photo Bleaching (FRAP) and Laurdan Generalized Polarization (GP). The study was performed on GUVs made of either phospholipids (POPC and DPPC) or amphiphilic copolymers based on Poly(dimethylsiloxane) and poly(ethyleneoxide). A significant deviation of stretching modulus, lateral diffusion coefficient and membrane packing was observed for GUVs formed by the PVA gel-assisted hydration method compared to the classical electroformation process. We were able to show that this deviation was due to the presence of PVA chains in the membrane and in the suspending medium. Globally our findings reveal that particular attention has to be paid on the gel assisted hydration process as the vesicles obtained present altered mechanical behaviour.Read less <
English Keywords
Lipid
Vesicle
Electroformation
membrane properties
polymer
Gel hydration
European Project
Erasmus Mundus - International Doctoral School in Functional Materials
ANR Project
Cinétique de Translocation de Particules à travers de Bicouches Auto-Assemblées - ANR-12-BS08-0018
Origin
Hal imported